OrangerV1, Main, Exploration, bibRecord, 001C86

Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs

Identifieur interne : 001C86 ( Main/Exploration ); précédent : 001C85; suivant : 001C87

Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs

Auteurs : Valeria E. Perotti [Argentine] ; Carlos M. Figueroa [Argentine] ; Carlos S. Andreo [Argentine] ; Alberto A. Iglesias [Argentine] ; Florencio E. Podesta [Argentine]

Source :

Plant science : (Limerick) [ 0168-9452 ] ; 2010.

RBID : Pascal:10-0481845

Descripteurs français

Pascal (Inist)
- Purification, Propriété physique, Cinétique, Fruit, Métabolisme, Biologie végétale, Citrus sinensis, Phosphoenolpyruvate carboxylase, Orange, Carbone.
Wicri :
- topic : Fruit, Carbone.

English descriptors

KwdEn :
- Carbon, Citrus sinensis, Fruit, Kinetics, Metabolism, Orange, Phosphoenolpyruvate carboxylase, Physical properties, Plant biology, Purification.

Abstract

Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C₃ plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.

Affiliations:

Argentine

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000215
to stream PascalFrancis, to step Curation: 000785
to stream PascalFrancis, to step Checkpoint: 000244
to stream Main, to step Merge: 001D28
to stream Main, to step Curation: 001C86

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs</title>
<author><name sortKey="Perotti, Valeria E" sort="Perotti, Valeria E" uniqKey="Perotti V" first="Valeria E." last="Perotti">Valeria E. Perotti</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquimicos and Facultad de Ciencias Bioquímicas y Farmacéuticas (CONICET-UNR), Suipacha 531</s1>
<s2>2000 Rosario</s2>
<s3>ARG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>2000 Rosario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Figueroa, Carlos M" sort="Figueroa, Carlos M" uniqKey="Figueroa C" first="Carlos M." last="Figueroa">Carlos M. Figueroa</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Laboratorio de Enzimologia Molecular, Instituto de Agrobiotecnología del Litoral (UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (UNL), Ciudad Universitaria</s1>
<s2>Paraje "El Pozo" S3000ZAA Santa Fe</s2>
<s3>ARG</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>Paraje "El Pozo" S3000ZAA Santa Fe</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Andreo, Carlos S" sort="Andreo, Carlos S" uniqKey="Andreo C" first="Carlos S." last="Andreo">Carlos S. Andreo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquimicos and Facultad de Ciencias Bioquímicas y Farmacéuticas (CONICET-UNR), Suipacha 531</s1>
<s2>2000 Rosario</s2>
<s3>ARG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>2000 Rosario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Iglesias, Alberto A" sort="Iglesias, Alberto A" uniqKey="Iglesias A" first="Alberto A." last="Iglesias">Alberto A. Iglesias</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Laboratorio de Enzimologia Molecular, Instituto de Agrobiotecnología del Litoral (UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (UNL), Ciudad Universitaria</s1>
<s2>Paraje "El Pozo" S3000ZAA Santa Fe</s2>
<s3>ARG</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>Paraje "El Pozo" S3000ZAA Santa Fe</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E." last="Podesta">Florencio E. Podesta</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquimicos and Facultad de Ciencias Bioquímicas y Farmacéuticas (CONICET-UNR), Suipacha 531</s1>
<s2>2000 Rosario</s2>
<s3>ARG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>2000 Rosario</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">10-0481845</idno>
<date when="2010">2010</date>
<idno type="stanalyst">PASCAL 10-0481845 INIST</idno>
<idno type="RBID">Pascal:10-0481845</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000215</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000785</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000244</idno>
<idno type="wicri:doubleKey">0168-9452:2010:Perotti V:cloning:expression:purification</idno>
<idno type="wicri:Area/Main/Merge">001D28</idno>
<idno type="wicri:Area/Main/Curation">001C86</idno>
<idno type="wicri:Area/Main/Exploration">001C86</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs</title>
<author><name sortKey="Perotti, Valeria E" sort="Perotti, Valeria E" uniqKey="Perotti V" first="Valeria E." last="Perotti">Valeria E. Perotti</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquimicos and Facultad de Ciencias Bioquímicas y Farmacéuticas (CONICET-UNR), Suipacha 531</s1>
<s2>2000 Rosario</s2>
<s3>ARG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>2000 Rosario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Figueroa, Carlos M" sort="Figueroa, Carlos M" uniqKey="Figueroa C" first="Carlos M." last="Figueroa">Carlos M. Figueroa</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Laboratorio de Enzimologia Molecular, Instituto de Agrobiotecnología del Litoral (UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (UNL), Ciudad Universitaria</s1>
<s2>Paraje "El Pozo" S3000ZAA Santa Fe</s2>
<s3>ARG</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>Paraje "El Pozo" S3000ZAA Santa Fe</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Andreo, Carlos S" sort="Andreo, Carlos S" uniqKey="Andreo C" first="Carlos S." last="Andreo">Carlos S. Andreo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquimicos and Facultad de Ciencias Bioquímicas y Farmacéuticas (CONICET-UNR), Suipacha 531</s1>
<s2>2000 Rosario</s2>
<s3>ARG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>2000 Rosario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Iglesias, Alberto A" sort="Iglesias, Alberto A" uniqKey="Iglesias A" first="Alberto A." last="Iglesias">Alberto A. Iglesias</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Laboratorio de Enzimologia Molecular, Instituto de Agrobiotecnología del Litoral (UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (UNL), Ciudad Universitaria</s1>
<s2>Paraje "El Pozo" S3000ZAA Santa Fe</s2>
<s3>ARG</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>Paraje "El Pozo" S3000ZAA Santa Fe</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E." last="Podesta">Florencio E. Podesta</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centro de Estudios Fotosintéticos y Bioquimicos and Facultad de Ciencias Bioquímicas y Farmacéuticas (CONICET-UNR), Suipacha 531</s1>
<s2>2000 Rosario</s2>
<s3>ARG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Argentine</country>
<wicri:noRegion>2000 Rosario</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant science : (Limerick)</title>
<title level="j" type="abbreviated">Plant sci. : (Limerick)</title>
<idno type="ISSN">0168-9452</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant science : (Limerick)</title>
<title level="j" type="abbreviated">Plant sci. : (Limerick)</title>
<idno type="ISSN">0168-9452</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon</term>
<term>Citrus sinensis</term>
<term>Fruit</term>
<term>Kinetics</term>
<term>Metabolism</term>
<term>Orange</term>
<term>Phosphoenolpyruvate carboxylase</term>
<term>Physical properties</term>
<term>Plant biology</term>
<term>Purification</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Purification</term>
<term>Propriété physique</term>
<term>Cinétique</term>
<term>Fruit</term>
<term>Métabolisme</term>
<term>Biologie végétale</term>
<term>Citrus sinensis</term>
<term>Phosphoenolpyruvate carboxylase</term>
<term>Orange</term>
<term>Carbone</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Fruit</term>
<term>Carbone</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C<sub>3</sub>
 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.</div>
</front>
</TEI>
<affiliations><list><country><li>Argentine</li>
</country>
</list>
<tree><country name="Argentine"><noRegion><name sortKey="Perotti, Valeria E" sort="Perotti, Valeria E" uniqKey="Perotti V" first="Valeria E." last="Perotti">Valeria E. Perotti</name>
</noRegion>
<name sortKey="Andreo, Carlos S" sort="Andreo, Carlos S" uniqKey="Andreo C" first="Carlos S." last="Andreo">Carlos S. Andreo</name>
<name sortKey="Figueroa, Carlos M" sort="Figueroa, Carlos M" uniqKey="Figueroa C" first="Carlos M." last="Figueroa">Carlos M. Figueroa</name>
<name sortKey="Iglesias, Alberto A" sort="Iglesias, Alberto A" uniqKey="Iglesias A" first="Alberto A." last="Iglesias">Alberto A. Iglesias</name>
<name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E." last="Podesta">Florencio E. Podesta</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C86 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C86 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:10-0481845
   |texte=   Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs

Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit iuice sacs

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri